Biotechnology, Health, News August 13, 2020

Engineered COVID-19-Infected Mouse Bites Researcher Amid ‘Explosion’ Of Risky Coronavirus Research

by Jonathan Latham

by Jonathan Latham, PhD

University researchers genetically engineer a human pandemic virus. They inject the new virus into a laboratory mouse. The infected mouse then bites a researcher…..It is a plot worthy of a Hollywood blockbuster about risky coronavirus research.

But according to newly obtained minutes of the Institutional Biosafety Committee (IBC) of the University of North Carolina (UNC), Chapel Hill, these exact events need not be imagined. They occurred for real between April 1st and May 6th this year.

The identity of the bitten coronavirus researcher has not been revealed except that they were working in a high security BSL-3 virus lab when the accident happened.

According to Richard Ebright, an epidemiologist from Rutgers University, the UNC incident underscores an important development in virus research since the pandemic began:

“There has been an explosion of research involving fully infectious SARS-CoV-2 over the last six months.  Research with infectious SARS-CoV-2 now is occurring in every, or almost every, BSL-3 facility in the US and overseas.”

This strong upsurge is affirmed by Edward Hammond of Prickly Research, Austin, TX, former Director of the Sunshine Project, an NGO that tracked the post 9/11 expansion of the US Biodefense program.

“It is evident that swarms of academic researchers with little prior experience with coronaviruses have leapt into the field in recent months.”

For Hammond, this explosion represents a hazard:

“We need to be clear headed about the risk. The first SARS virus was a notorious source of laboratory-acquired infections and there is a very real risk that modified forms of SARS-CoV-2 could infect researchers, especially inexperienced researchers, with unpredictable and potentially quite dangerous results. The biggest risk is the creation and accidental release of a novel form of SARS-CoV-2 — a variant whose altered characteristics might undermine global efforts to stop the pandemic by evading the approaches being taken to find COVID vaccines and treatments.”

And, continues Hammond: “Each additional lab that experiments with CoV-2 amplifies the risk.”

Richard Ebright concurs, telling Independent Science News in an email that this research is:

“in many cases being performed by researchers who have no prior experience in BSL-3 operations and pathogens research, and who therefore pose elevated risk of laboratory accidents with BSL-3 pathogens.”

Ebright is also concerned that some influential experimenters are now calling for reduced oversight :

“The UNC incident also underscores that calls by some, notably Columbia University virologist Vincent Racaniello (Podcast at 01:35mins onwards), to allow virus-culture and virus-production research with fully infectious SARS-CoV-2 at BSL-2 are egregiously irresponsible and absolutely unacceptable.”

Other researchers are also calling for restraint. In a paper titled “Prudently conduct the engineering and synthesis of the SARS-CoV-2 virus”, researchers from China and the US critiqued the synthesis in February of a full length infectious clone (Gao et al., 2020; Thao et al., 2020). And, in concluding, these researchers asked a question that is even more pertinent now than then “Once the risks [of a lab escape] become a reality, who or which organization should take responsibility for them?”

Lack of transparency

The accident at the University of North Carolina (UNC) is now in the public domain but only thanks to a FOIA request submitted by Hammond (in line with NIH guidelines) and shared with Independent Science News.

Despite the FOIA request, apart from the fact that UNC classified it as an official “Reportable Incident”, i.e. that must be reported to National Institutes of Health (NIH) in Washington DC, scarcely any information about the accident is available.

In part this is because the minutes of the relevant IBC meeting (May 6th, 2020, p109) are extremely brief. They do not provide any details of the fate of the bitten researcher. Nor do they state, for example, whether the researcher developed an active infection, nor whether they developed symptoms, nor if they transmitted the recombinant virus to anyone else. Neither do they reveal what kind of recombinant virus was being used or the purpose of the experiment.

To try to learn more, Independent Science News emailed the lab of Ralph Baric at UNC, which, based on their research history is the most likely coronavirus research group involved (Roberts et al., 2007; Menachery et al., 2015), the University Biosafety Officer, and UNC Media relations.

Only the latter replied:

“The April 2020 incident referred to in the University Institutional Biosafety Committee meeting minutes involved a mouse-adapted SARS-CoV-2 strain used in the development of a mouse model system.”

Ralph Baric UNC Gillings School of Public Health-web
Ralph Baric UNC Gillings School of Public Health-web

UNC media relations also told Independent Science News that:

“The researcher did not develop any symptoms and no infection occurred as a result of the incident.”

Our questions in full and the full UNC reply are available here.

Redactions of Biosafety Committee discussions

The second reason for this lack of information is that the UNC redacted the names of Principal Investigators (PIs) whose research required biosafety scrutiny, along with many of the experimental specifics.

Nevertheless, unredacted parts of minutes from IBC meetings held in 2020 contain descriptions of experiments that potentially encompass the accident. They include:

Application 75223:

UNC Inst Biosafety Committee Application 75223
UNC Inst Biosafety Committee Application 75223

(“a full-length infectious clone” refers to a viable DNA copy of the coronavirus, which is ordinarily an RNA virus)

and

Application 73790:

UNC Inst Biosafety Committee Application 73790
UNC Inst Biosafety Committee Application 73790

and

Application 74962:

UNC Inst Biosafety Committee Application 74962
UNC Inst Biosafety Committee Application 74962

In all, any one of eight sets of different experiments approved by the UNC Chapel Hill IBC in 2020 proposed infecting mice with live infectious and mutant SARS-CoV-2-like coronaviruses under BSL-3 conditions and therefore could have led to the accident.

The thorny issue of transparency

According to Hammond the lack of transparency represented by the sparse minutes and especially the redactions represent a violation of science’s social contract:

“At the dawn of recombinant DNA, at the request of the scientific community itself, following the fabled Asilomar conference, the United States government took the position of not regulating genetic engineering in the lab.  The “deal” that big science struck with the government was that, in return for not being directly regulated, principal investigators would take personal responsibility for lab biosafety, involve the public in decision-making, and accept public accountability for their actions.

The NIH Guidelines and Institutional Biosafety Committee system of “self-regulation” by researchers is founded upon the principal of personal responsibility of PIs and the promise of transparency.  The redaction of the researchers’ identities from IBC meeting minutes, in order to hide the activities of researchers and avoid accountability for accidents, fundamentally contradicts the core principles of the US oversight system and violates the commitments that science made.”

Richard Ebright goes further:

“There is no justification for UNC’s redaction of the names of the laboratory heads and the identities of pathogens.  UNC’s redactions violate conditions UNC agreed to in exchange for NIH funding of UNC’s research and, if not corrected, should result in the termination of current NIH funding, and the loss of eligibility for future NIH funding, of UNC’s research.”

Are universities doing too many risky experiments on coronaviruses?

The second concern of researchers contacted by Independent Science News is that unnecessary and dangerous experiments will be conducted as a result of the COVID-19 pandemic. According to Richard Ebright:

“The UNC incident shows that serious laboratory accidents with SARS-CoV-2 can occur even in a lab having extremely extensive experience in BSL-3 operations and unmatched expertise in coronavirus research, and underscores the risks associated with uncontrolled proliferation of research on SARS-CoV-2, especially for labs lacking prior experience in BSL-3 operations and coronavirus research.”

For this reason Ebright argues that:

“It is essential that a national needs-assessment and biosafety assessment be performed for research involving fully infectious SARS-CoV-2.  It also is essential that a risk-benefit review be performed before approving research projects involving fully infectious SARS-CoV-2–something that currently does not occur–to ensure that potential benefits to the public outweigh the real risks to laboratory workers and the public.”

This concern over risks and benefits is shared by Edward Hammond. Using FOIA again he has further discovered that researchers at the University of Pittsburgh (whose identity is redacted) plan to make what Hammond calls Corona-thrax.

In short, according to its Institutional Biosafety Committee, Pittsburgh researchers intend put the spike protein of SARS-CoV-2 (which allows the virus to gain entry into human cells) into Bacillus anthracis which is the causative agent of anthrax.

U Pittsburgh Inst Biosafety Committee minutes 2020
U Pittsburgh Inst Biosafety Committee minutes June 2020

The anthrax strain proposed to be used for this experiment is “disarmed” but, Hammond agrees with Gao et al., (2020) that the balance of risks and benefits appears not to be receiving adequate consideration.

This experiment was nevertheless approved by the Institutional Biosafety Committee of the University of Pittsburgh. But by redacting the name of the laboratory from the minutes and also every name of the members of the committee which approved it, the University has supplied a de facto response to the final question posed by Gao et al.: who will take responsibility for risky coronavirus research?

References

Gao, P., Ma, S., Lu, D., Mitcham, C., Jing, Y., & Wang, G. (2020). Prudently conduct the engineering and synthesis of the SARS-CoV-2 virusSynthetic and systems biotechnology5(2), 59-61.
Menachery, V. D., Yount, B. L., Debbink, K., Agnihothram, S., Gralinski, L. E., Plante, J. A., … & Randell, S. H. (2015). A SARS-like cluster of circulating bat coronaviruses shows potential for human emergence. Nature medicine21(12), 1508-1513.
Roberts, A., Deming, D., Paddock, C. D., Cheng, A., Yount, B., Vogel, L., … & Zaki, S. R. (2007). A mouse-adapted SARS-coronavirus causes disease and mortality in BALB/c mice. PLoS Pathog3(1), e5.
Thao, T. T. N., Labroussaa, F., Ebert, N., V’kovski, P., Stalder, H., Portmann, J., … & Gultom, M. (2020). Rapid reconstruction of SARS-CoV-2 using a synthetic genomics platformBioRxiv.

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Comments 5
  • totally crazy. what for??

    Keep up the great investigative science-journalism.

  • When I was working for Friends of the Earth, we filed a lawsuit against several government agencies because they refused to prepare an environmental impact statement (as required by NEPA) before promulgating research guidelines. FOE had hoped to pursue litigation but could not find scientists willing to be expert witnesses. There were too many conflicts of interest. Scientists who strongly favored following the law were almost nonexistent. One of these was the late Marc Lappe, who was physically pushed against a wall by another scientists for questioning the refusal of scientists to accept regulation and follow the law regarding an EIS.
    FOE”s president, Dave Brower, was himself besieged by “friends” to drop the lawsuit but refused. In our NY office Francine Simring established the Committee for Responsible Genetics, with globally notable credible scientists such as Erwin Chargaff and many others, urging a slow down in research and a commitment to preparing a comprehensive EIS, etc.
    But it was an uphill battle and FOE was not able to raise enough money to continue the lawsuit.

  • The social contract that science is supposed to have had with society was dissolved long back . It exists today only in fictional children stories .
    Modern science has entered the realm of fascism now.
    The race to patent is pramount
    In the field of agriculture science encourages a kind of ethnic cleansing , and we farmers are encouraged to dump the environmental costs back on the environment for the future to pay .
    This was not the promise science made ….it is the con job carried out by the corporation’s .
    Article was well written, need more transparency
    Best wishes and regards .
    Vivek Cariappa

  • I second the opinion of vivek above.

    It seems that to be an honest scientist, on the side of humanity, you must now also fight crime like a superhero.

  • The comments stated above are on target. I was employed at the USEPA for 16 years before being forced out for doing my job as per my job description. Thus, I concur with comments stated above.

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