by Jonathan Latham, PhD and Allison Wilson, PhD
One of the very earliest scientific papers from the COVID-19 pandemic era now has over 11,000 citations. Appearing in the scientific journal Nature on February 3rd 2020, Zhou et al., 2020 reported the genome sequence of a novel coronavirus isolated from patients with atypical pneumonia in Wuhan, China. Its senior author was leading coronavirus researcher Zheng-li Shi of the Wuhan Institute of Virology (the WIV). Along with what we now call SARS-CoV-2, her paper also reported the genome sequence of a closely related (96.2% identical) bat virus. The authors called this virus RaTG13. To this day RaTG13 is still the closest known viral genome by far to SARS-CoV-2.
RaTG13 came from the freezers of the WIV.
In one sense, there is nothing unusual about that. Researchers often have old samples stored away and the WIV has the largest collection of coronaviruses in the world. On the other hand, Wuhan is considered an unlikely locality for a coronavirus outbreak.
However, the two sentences in Zhou et al., (2020) describing RaTG13 allayed any fears:
“We then found that a short region of RNA-dependent RNA polymerase (RdRp) from a bat coronavirus (BatCoV RaTG13)–which was previously detected in Rhinolophus affinis from Yunnan province–showed high sequence identity to 2019-nCoV. We carried out full-length sequencing on this RNA sample (GISAID accession number EPI_ISL_402131).”
This statement clearly implied that, prior to the pandemic, WIV researchers had only ever studied a ‘short’ fragment of RaTG13, that the fragment was unpublished (since no prior description was cited), and that no recent research had been carried out on RaTG13 or its sample. Consequently, a lab leak involving RaTG13 was implausible.
But all these inferences turned out to be untrue. Zhou et al. was one of the pandemic’s key misinformation superspreaders. In August 2020, two researchers, Monali Rahalkar and Rahul Bahulikar, published a preprint in which they reported that they had accessed the underlying sequencing files for RaTG13. As first noticed by Twitter user Francisco A. de Ribeira, some were dated from 2017 and others from 2018. Moreover, these early RaTG13 sequences went far beyond a short fragment, encompassing most of the genome (Rahalkar and Bahulikar, 2020a).
In November 2020 Nature published an addendum to Zhou et al. in which Zheng-li Shi and her co-authors conceded these points. They wrote:
“In 2018…..we performed further sequencing of these bat viruses and obtained almost the full-length genome sequence (without the 5′ and 3′ ends) of RaTG13.”
Thus the RaTG13 genome sequence was mostly deciphered in 2018 and not in January 2020. Ergo, the viral genome most similar to SARS-CoV-2 was being studied at the WIV before the pandemic broke out.
But why hide this activity?
The outbreak in the Mojiang Mine
By the time of these revelations, yet other questions had arisen about RaTG13. It turned out that RaTG13 was an exact genetic match to an already published partial virus sequence called BtCoV/4991. BtCoV/4991 had been obtained by Zheng-li Shi’s group in 2013 from a mine in Yunnan Province, China (Ge et al., 2016).
Furthermore, RaTG13 and BtCoV/4991 were from the same bat anal swab sample. In other words, from the data provided, the two were indistinguishable. Thus, RaTG13 did have a publishing history, but under the name BtCoV/4991 (Bengston, 2020).
In addition to BtCoV/4991 another novel betacoronavirus was found at the same time. Their discoverers concluded the following:
“Considering that the two highly pathogenic human coronaviruses (SARS-CoV and MERS-CoV) in this genus [the Betacoronaviruses] originated from bats (Ge et al., 2013; Lau et al., 2013; Corman et al., 2014), attention should be particularly paid to these lineages of bat coronaviruses.” (Ge et al., 2016)
According to their own words, it would therefore be surprising had Zheng-li Shi’s group not gone on to study BtCoV/4991 aka RaTG13.
Yet the discovery of this publication trail only deepened the mystery yet further. Though never mentioned by Ge et al., the ‘abandoned’ mine (which subsequently became known as the Mojiang mine) where BtCoV/4991 was found had recently been the site of a mystery disease outbreak. In April 2012, just two and a half months before the first WIV sampling trip, six miners had become sick and three of them had died. Indeed, the mine outbreak was presumably why the WIV researchers were sampling there (and the Zhou et al. addendum later confirmed this).
The nature of the 2012 disease outbreak became much clearer with the discovery (by an anonymous Twitter user called @TheSeeker268) of a 2013 Chinese Master’s thesis. This thesis is titled “The Analysis of Six Patients With Severe Pneumonia Caused By Unknown Viruses“. Its abstract specifically mentions a possible outbreak of SARS-like coronaviruses.
To learn more about the 2012 pneumonia outbreak, we arranged to have the Master’s thesis translated into English.
The translation yielded a cornucopia of information. This began with the fact that the author of the thesis was the doctor who supervised the testing and treatment of the miners at the First Affiliated hospital of Kunming Medical University (Yunnan Province).
According to the Master’s thesis, the miners had been shovelling bat guano. This implied that the mystery disease probably originated from bats. That, along with various test results and consultations led the author to conclude that the most likely cause of the outbreak was a coronavirus. But perhaps most startling of all the findings to emerge from the translation was that the symptoms of the miners closely resembled those of COVID-19 (Rahalkar and Bahulikar, 2020b).
The Master’s thesis thus raised a host of questions. Why did the Ge et al. 2016 publication (from Zheng-li Shi’s lab), which sampled the mine on multiple occasions, make no mention of the outbreak that caused them to be sampling there in the first place? Why did Zheng-li Shi tell Scientific American in March 2020 that the miners died of a fungal infection? Why did her Zhou et al. 2020 publication rename BtCov/4991 as RaTG13 without explanation or even referencing the earlier work on BtCov/4991 or its mine origin? Especially, given that Zheng-li Shi and her corona-colleagues in China and elsewhere have constantly warned of coronavirus spillovers from bats (e.g. Zhou et al., 2018), why did no one seemingly prove or disprove the coronavirus theory in the Master’s thesis? Are we to presume that an apparent textbook case of a zoonotic spillover, significant enough at the time to be covered by Science magazine, was in the end simply ignored?
So many interrelated errors and omissions present a conundrum. Are these concatenated oversights simply innocent happenstances? Or were they deliberate attempts to obscure the connections from RaTG13 back to BtCov/4991 and from there to the mine and thence to the mine outbreak; to thereby prevent anyone in 2020 linking SARS-CoV-2 and the mine outbreak in 2012? Is there something about the mine or the miners of deep significance to the current pandemic? If so, what?
The value of the PhD thesis
Given that the broader context of the pandemic origin is the failure of a plausible zoonotic origin scenario to emerge (Quay, 2021; Segreto et al., 2021), we have attempted to resolve this conundrum by proposing the Mojiang Miners Passage theory of the origin of SARS-CoV-2.
The theory was stimulated chiefly by two observations. The first was that some of the miners with the mystery disease were ill for about five months (April to September, 2012). The second key observation was the assertion in the Master’s thesis that diagnostic samples taken from four of the miners were sent to the Wuhan Institute of Virology.
What we proposed, in short, is that the miners contracted one, or maybe multiple, RaTG13-like bat coronaviruses in the mine. The long hospitalization of the miners enabled the initial bat virus to evolve into a human coronavirus by providing conditions similar to extended viral passaging. The diagnostic samples sent to the WIV therefore contained a humanised virus and we proposed it was similar, or even identical, to SARS-CoV-2. Virus isolation, or virus culture in human or other cells, or perhaps even Gain-of-Function type experiments, then permitted this virus to later escape from the WIV in late 2019.
Given all this, a very significant unresolved question becomes: What tests were done on the samples taken from the miners and sent to the WIV? What were the results? The answers are important not only for the Mojiang Miners Passage theory but also for other explanations of the pandemic origin. For example, other lab escape theories have proposed that RaTG13 (or a similar unpublished genome) was the likely backbone for Gain-of-Function research (see Sirotkin and Sirotkin, 2020; Segreto and Deigin, 2020; Segreto et al., 2021; Kaina, 2021). But it is also possible that a virus extracted from the miners was the starting point.
According to the Master’s thesis, Prof. Nanshan Zhong advised SARS testing and samples from four miners were therefore sent to the WIV. These tested positive for coronavirus in an IgM antibody test (See Fig. 1).
In their November addendum Zheng-li Shi and her colleagues at the WIV confirmed receiving thirteen serum samples–taken at approximately monthly intervals in 2012–from four of the hospitalised miners. But here there is a major discrepancy. Referring to the miner serum samples, the addendum states:
“To investigate the cause of the respiratory disease, we tested the samples using PCR methods developed in our laboratory targeting the RNA-dependent RNA polymerases of Ebola virus, Nipah virus and bat SARSr-CoVRp3, and all the samples tested negative for these viruses. We also tested the serum samples for the presence of antibodies against the nucleocapsid proteins of these three viruses, and none of the samples gave a positive result. Recently, we retested the samples with our validated enzyme-linked immunosorbent assay (ELISA) against the SARS coronavirus 2 (SARS-CoV-2) nucleocapsid protein–which has greater than 90% amino acid sequence identity with SARSr-CoVRp3–and confirmed that these patients were not infected by SARS-CoV-2.” (Zhou et al., 2020, addendum)
The implication is that, though various tests were performed at the WIV, SARS-CoV-2 was not found and no evidence for any other coronavirus was found either.
This obviously contradicts the Master’s thesis.
The difference between these two positions is crucial. If a coronavirus of any kind was found in the miners’ samples it would likely alter our whole understanding of the pandemic origin.
This is because every possible effort would surely have been made to identify it, grow it in cultured human cells, sequence its genome, characterise its infectivity, and much more. There are two primary reasons for this.
First, discovering a coronavirus infection in the miners would triumphantly vindicate the basic research premise of Zheng-li Shi and the WIV. Their pitch is that bat coronaviruses are dangerous pathogens that may at any moment jump to humans and other animals.
Second, a novel coronavirus in the miners would represent a veritable research bonanza: a highly lethal zoonotic disease outbreak caught in the act.
By the same token, if a coronavirus was found in the 2012 miners’ samples it would surely also mean that subsequent research, presumably at the WIV, has been concealed.
This then is the background to our interest in any credible document containing further evidence that the research trail from the miners did not go cold after the 2012 outbreak.
The PhD thesis of Canping Huang
For many months, a scientific document has been shared on the internet that appears to shed much light on the fate of samples taken from the mine and the miners, as well as the broader context. It is a translation of a Chinese PhD thesis originally written in 2016. Like the Master’s thesis it also was found on the Chinese thesis database by @TheSeeker268. Its English title is “Novel Virus Discovery in Bat and the Exploration of Receptor of Bat Coronavirus HKU9”. (Download the Chinese PhD thesis here)
The author of this thesis is Canping Huang, who was a student of Gao Fu. Gao Fu is an important virologist known in the West as George Gao. In 2017 he was appointed head of China’s Center for Disease Control and Prevention, China’s CDC.
The direct relevance of the Canping Huang thesis, however, is that the author is one of relatively few researchers documented to have sampled the Mojiang mine for bat viruses.
Previously, a provisional and partial translation of this thesis was generated, using Google translate, by the previously mentioned Francisco A. de Ribera. Using this earlier version as a guide, we have organised a professional and expanded translation.
This new version focuses on the parts of the thesis most relevant to the origin story of SARS-CoV-2; in particular, Chapter 3 titled: Initial investigation of the causes of unexplained severe pneumonia incidents related to an abandoned mining cave found in Mojiang Hani Autonomous County, Yunnan Province.
The Canping Huang thesis substantiates several aspects of the Mojiang Miners Passage theory. But perhaps the most significant of all its statements is that samples from four of the miners were tested at the WIV for IgG antibodies against the SARS virus. According to the PhD (see Fig. 3), all samples were positive. (This test is distinct from the IgM test mentioned in the Master’s thesis).
The logical interpretation of an IgG antibody test for SARS giving a positive result in 2012 is that the miners were infected with a coronavirus, presumably a SARS-related one. It is highly unlikely, however, that the miners had SARS itself, since the SARS virus had not been observed since 2003. Moreover, the original SARS outbreak was in Guangdong province, far from the Mojiang mine. Thus, the miners presumably were infected with a virus similar, but not identical to, SARS, i.e. a novel coronavirus. Most importantly, this is the conclusion reached also by the Master’s thesis (see Fig 4).
What adds particular credibility to the PhDs’ claim are its specifics. According to Canping Huang, of the four miners:
“two discharged patients had higher antibody levels, whereas two hospitalized patients had lower antibody levels”.
Such details imply that the PhD’s assertion of positive SARS tests is not a simple mistake, nor based on mere hearsay. Rather, as the mysterious pneumonia outbreak was author’s reason to sample the mine (see Chapter 3.), these results had crucial significance to the thesis; just as they would have had to his supervisor and the Chinese CDC. Furthermore, a PhD is a peer-reviewed document. It has to be accepted by the supervisor and a committee. If the tests and/or the positive results were cited in error then these were multiple errors with multiple parents.
Indeed, in an interview with French TV (at 18:23), George Gao was asked about the antibody tests of the miners and he confirmed the positive tests.
Since it is not easy to dismiss, this assertion of multiple positive tests is a fact of great significance. As we and others have suggested, it seems likely that the miners had COVID-like symptoms because they were infected with a SARS-like coronavirus.
If so, researchers at the WIV have attempted to hide that diagnosis.
Is it time for an addendum to the addendum?
Ideally, one would seek to clarify precisely where the assertions in the PhD thesis (positive IgG test of four patients for SARS) and the Master’s thesis (positive IgM tests for an unspecified number of patients) contradict those in the Zhou et al. addendum.
However, although it purports to resolve the issues, the addendum is a complete mismatch with the questions raised by either thesis. Firstly, nowhere does the addendum positively state that no virus or no coronavirus was found in the miners’ serum samples.
Secondly, the addendum does not mention a SARS test. It mentions instead two different tests for SARSr-CoV-Rp3 (a bat virus related to SARS), and one test for SARS-CoV-2. It is presumably possible that the PhD and the Master’s and George Gao were mistaken and conflated tests for SARSr-CoVRp3 with ones for SARS itself (or perhaps were misinformed); but this does not alter the situation fundamentally since, contra the addendum, all three sources claim the results were positive.
Failure to directly address the principal questions spotlights a noteworthy additional defect of the addendum. It lacks the key accoutrements of a scientific publication. The addendum asserts the results of three tests but their methods are not fully described, nor are the materials and reagents. The results, including controls, are also not shown. Importantly too, the eight similar viruses mentioned as being found along with RaTG13/4991 are neither referenced nor specified by name, even though their genomes may be crucial for tracing the pandemic’s origins. It is unscientific and ultimately somewhat ridiculous for Nature to publish an addendum making controversial, claims that are crucial to understanding the origin of a global pandemic costing millions of lives, but make meaningful independent scrutiny of those claims impossible.
Nevertheless, the addendum should raise yet more eyebrows. When Canping Huang identified and isolated novel viruses from his bat samples, the methods he used, in Chap 3., were pan-coronavirus primers in PCR experiments and viral metagenomics in the form of Next Generation Sequencing (NGS). Yet neither method is mentioned in the addendum. The tests described by the addendum are not worthless, but they are also not best practice when the question is how to detect a mystery coronavirus. Apparently, the WIV, a world-leading institute, did not apply the most sensitive or effective available methods in this crucial and unprecedented instance of a zoonotic jump caught in flagrante.
China is a leader in gene sequencing technology but one could perhaps imagine that in 2012-13 NGS was unavailable to Zheng-li Shi. However, we know that she sent samples to Beijing for NGS as early as 2011 (Ge et al., 2012). In any case, it is available today. When Zheng-li Shi’s group first identified the novel SARS-CoV-2 virus from patients in Wuhan they used pan-coronavirus primers. Next they used metagenomic sequencing to decipher its genome (Zhou et al., 2020). We know too that the miners’ samples are apparently not depleted since the addendum claims to have ‘retested’ them ‘Recently’.
Additionally, there are now powerful methods for retrieving degraded and low abundance genetic material, even from biological samples millions of years old (e.g. Dabney et al., 2013). In 1997 the Spanish Flu influenza genome was reconstructed from preserved human tissues (Taubenberger et al., 1997). If necessary, such methods could surely be applied to any of the extant samples from the miners.
The PhD thesis translation prompts still further questions about the addendum and the miners’ samples, For example, we need to know more about the 13 serum samples. What dates were they taken on and which miners did each come from? Also, the Master’s thesis mentions extraction of the thymus of patient 4, while both it and the PhD mention that throat swabs were taken (PhD, p. 82). According to both theses, such samples were sometimes sent to other institutions. In many cases, these samples probably represent better opportunities to collect a coronavirus and demonstrate a virus spillover than does a serum sample. Did the WIV receive or request other samples? Were tests performed on them? If so, what were the results? Answers, one hopes, along with data to support them, will perhaps be provided in a second addendum.
In the final analysis this situation is ultimately quite simple: three sources (the Master’s thesis, Canping Huang’s PhD and George Gao) clearly and credibly contradict the implication of the addendum on the key question of whether a coronavirus was detected in the miners’ serum samples. Moreover, two of these sources predate the pandemic. Unlike the WIV authors, no case can reasonably be made that the two thesis authors were motivated by self-preservation as a consequence of the pandemic. The WIV authors, in contrast, have a track record of misleading statements and clear omissions in connection with the miners outbreak and RaTG13; hence the need for the original addendum.
Further revelations of the Canping Huang PhD thesis
The Canping Huang PhD contains other data and important statements. It corroborates the Master’s thesis, lends significant support to the Mojiang Miners Passage theory, and further contradicts the accounts of researchers from the WIV.
1) Canping Huang was a virologist. Together with a team assembled by the Chinese CDC, he searched the Mojiang mine specifically for viruses. In all, at least four independent teams of Chinese virologists (some representing multiple labs) searched the Mojiang mine.
Beginning in July 2012, researchers from Zheng-li Shi’s lab at the WIV visited the mine up to seven times (WHO report, Annex, p131). In it they found several hundred Alphacoronaviruses and ten Betacoronaviruses [the addendum states nine Betacoronaviruses but it appears to omit HpBtCoV/3740-2, which was also found there (Ge et al., 2016)] (Zhou et al., 2020 addendum). Of these, the Betacoronaviruses are the most significant because RaTG13 and SARS-CoV-2 are Betacoronaviruses. Later in 2012, researchers from Peking Union Medical College and the Institute of Pathogen Biology in Beijing also took samples. They identified a paramyxovirus in rats from the mine (Wu et al., 2014). The third team was a group from the University of Hong Kong who found Alphacoronaviruses in the mine (Lau et al., 2015). The fourth was Canping Huang in 2014. This intense sampling activity shows that prominent Chinese researchers like George Gao suspected that the causative agent of the mine outbreak was a virus, and were still hunting for clues in 2014. Nevertheless, the March 2021 WHO report, like the earlier Scientific American interview, attributes the miners’ illnesses to a fungal infection (WHO Annex, p131). However, it should be emphasized that, once again, this mention of a fungal origin by the WHO is not supported by any data or cited evidence. Nor is there any suggestion that fungal biologists investigated the outbreak. The case for a fungal outbreak is therefore very weak.
2) Canping Huang and his colleagues caught and killed 87 bats and one rat. This sampling bias suggests bats were still considered the most likely source of the miners’ disease in 2014.
3) In these bats, Canping Huang found four astroviruses, one bocavirus and two Alphacoronaviruses. This indicates that bats with coronaviruses continued to be present in the mine in 2014, two years after the outbreak. More importantly, his use of degenerate (i.e. all-purpose) PCR primers designed to amplify coronavirus genomes (see Chapter 3 of the PhD) demonstrates that Canping Huang was actively searching specifically for coronaviruses (and also for certain other viruses too).
4) The PhD also states that blood samples from the miners were sent to the Wuhan Institute of Virology. Prior to the November Zhou addendum, only the Master’s thesis made such a claim. Thanks to Canping Huang’s thesis, three independent sources agree that serum/blood samples were sent to the Wuhan Institute of Virology.
5) The thesis also confirms important statements in the Master’s thesis that samples from the infected miners were sent to laboratories across China. According to the PhD:
“In order to analyze the pathology and the cause of the disease, the Chinese Yunnan Centers for Disease Control and Prevention, other medical centers and other research labs collected samples from the patients, bats, rats and the environment. The blood samples of four cases showed that: four patients’ throat swabs and whole blood samples were negative for SARS coronavirus*, epidemic hemorrhagic fever, Dengue fever (type 1-4), epidemic encephalitis type B, flavivirus and alphavirus pathogen nucleic acid tests (Chengdu Military Region for Disease and Control). The blood samples of four of the cases, and from four other people who had been to the cave yet had no symptoms, all showed no abnormal results (Guangdong, lab of Nan Shan Zhong); The bats in the mine were dissected, and bat feces were tested, and no abnormality was found (Wuhan Institute of Virology, Chinese Academy of Sciences)……Half a year later, the Jin Qi lab went to the same cave to collect samples from bats and wild rats and tested for pathogens.”
*Authors note: A negative test for SARS, performed soon after admission of the miners to hospital, is also mentioned in the Master’s thesis, which largely discounts its results.
This quite lengthy list of interested laboratories goes beyond those listed in the Master’s thesis, but is not surprising. A lethal disease outbreak originating from bats with a probable viral aetiology is not an everyday occurrence. The previous one in China was declared a pandemic: the first SARS outbreak in 2002. Hence, top virology labs in China, such as those of George Gao, Zhong Nanshan, Zheng-li Shi and Qi Jin became involved in investigating the outbreak.
We are nevertheless left with a mystery, which the Canping Huang thesis deepens considerably. Why, given this strong interest, were only two quite minor scientific papers ever published that mentioned the mine, of which only one mentioned the outbreak (Wu et al., 2014; Ge et al., 2016)? Especially since no alternative cause for the outbreak was found.
Undisclosed research on emerging infectious diseases in China
Ever since the first SARS outbreak, Chinese virologists have very actively searched for potential human zoonotic pathogens, especially coronaviruses (e.g. Lau et al., 2015; Latinne et al., 2020). A “major project” called “Discovery of Zoonotic Pathogens and Study of its Pathogenicity to Humans” was funded by the National Science Foundation of China for this specific purpose. This programme was established in 2012, the year of the mine outbreak, and included Zheng-li Shi as one of its principle investigators.
There are strong signs that plenty of this emerging infectious coronavirus research remains unpublished, even those parts that are highly connected to the origins of the pandemic. For example, Chapter 3. of Canping Huang’s thesis was never published or otherwise made available. Though he identified two Alphacoronaviruses in the Mojiang mine and obtained partial genome sequences, he did not show them. They are not in the thesis itself nor does he seem to have deposited them in any public database.
A key example is that Zheng-li Shi also possesses unpublished coronavirus genomes from the mine. These include at least eight other very closely related Betacoronaviruses that she has featured in several recent oral presentations and for which she has at least partial genome sequences. These are called Ra7909, Rst7896, Rst7905, Rst7907, Rst7921, Rst7924, Rst7931, and Rst7952. These genomes, if published, would approximately double the number of published genomes in the SARS-CoV-2 phylogenetic clade, which is the phylogenetic group containing all the closest relatives of SARS-CoV-2. The information contained in these genomes would doubtless be extremely valuable for understanding the ancestry of SARS-CoV-2. It is therefore remarkable that they remain unpublished over a year after the Wuhan outbreak. Similar points can be raised about the more distantly related Betacoronavirus HpBtCoV/3740-2 also identified from the Mojiang mine by her group (Ge et al., in 2016). The only publicly available information on this virus is a short fragment deposited in a database five years ago.
Adding to this list of unpublished research is a very interesting recent preprint by a group of independent researchers. It is titled “Unexpected novel Merbecovirus discoveries in agricultural sequencing datasets from Wuhan, China” and it reports the existence of a series of hitherto unpublished full- and partial-length Betacoronavirus genome sequences (Zhang et al., 2021). These genomes were uncovered by examining Chinese sequencing datasets (mostly originating in Wuhan) uploaded to the NCBI genome repository. These datasets were then searched by the authors for coronavirus sequence contamination. Coronavirus sequence reads found in these datasets were then assembled by the researchers into whole or partial coronavirus genomes. What such searches reveal are either instances of biological contamination (e.g. stray viruses in biological cultures or whole organisms) or stray sequences resulting from methodological contamination during sequencing–usually sample mix-ups of a kind known as index hopping.
Among the findings made by these researchers were a full-length novel HKU5-related coronavirus and an apparent full-length infectious clone of a novel HKU4-related coronavirus. These are both MERS-related coronaviruses. These were not the only coronaviruses they found and therefore what the data suggests is that, in China, numerous labs are isolating, culturing, or otherwise studying unpublished coronaviruses.
These examples of unpublished viruses may only be the tip of an iceberg. What they and the examples that preceded it highlight, however, is the importance of accessing the WIV’s virus databases that were taken offline at the outset of the pandemic. These are where unpublished viral sequences from the WIV and elsewhere were stored. The need to know which viruses closely related to SARS-CoV-2 were being studied by scientists in Wuhan in 2019 is urgent.
Future directions for lab origin investigations
Given the pattern of evidence that is accumulating it is encouraging that members of the US Congress have written to Peter Daszak of the EcoHealth Alliance with a list of key questions about the relationship of the EcoHealth Alliance with the WIV. This letter in particular requests access to virus samples held by them and especially those samples related to the Mojiang mine at the time of the outbreak (see Fig. 5).
The EcoHealth Alliance has had funding and active working relationships with coronavirus researchers at the WIV for over a decade. Given the obstructive role it played in resisting investigations of laboratory origins, any effort to get answers from the EcoHealth Alliance deserves wide support. The Congressional letter is constructive in that it combines a precise focus on the key scientific data gaps with a broad view of accountability. In the final analysis, exactly who might have spilled the SARS-CoV-2 virus is not necessarily more important to know than who funded them and who conceived and instigated the research.
The Congressional letter raises many questions pertinent to any investigation of the virus’ origin. What is altered by the revelations of the PhD is to render much more compelling the probability, first asserted in the Master’s thesis, that there was a coronavirus in medical samples taken from the miners. This in turn serves to underline the obvious inconsistencies and omissions of Zhou et al. 2020 and especially its addendum. These flaws make the likelihood that the Mojiang mine played a key role in the COVID-19 outbreak seem ever more plausible and the need to obtain samples and sequences still more urgent.
All origin possibilities should be considered and fully investigated. But in our view, the Mojiang Miners Passage origin theory is particularly valuable because it is a scientifically testable hypothesis. If a transparent and independent analysis of samples from each of the miners were carried out it should show whether or not they had a coronavirus infection. If present in the samples a coronavirus genome sequence (even a quite short fragment) would likely definitively prove, or disprove, the theory beyond any reasonable doubt.
According to the WHO origin report, Chinese virologists have scoured the country for bat-derived coronaviruses. But at the same time, the WIV and the EcoHealth Alliance have not shared what is apparently in their own freezers and databases. The obstacle therefore seems not to be one of ability to share data but of willingness. But such obvious reticence carries a risk. The less openness scientists at the WIV and the EcoHealth Alliance show and the more they appear to dodge the key questions, the more the suspicion will unavoidably grow of their collective culpability for whatever really happened in Wuhan in late 2019.
The authors would like to acknowledge the generous assistance of Francisco A. de Ribera in preparing this article.
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On the fungus cause-of-illness/death, noting that a fungus is attacking immune-compromised covid-19 patients (diabetic/high steroid use) in India, this from the BBC:
I think I need to read that again, but the way it all came together, in the absence of any of the other teams of researchers finding something similar in the mine, juxtaposed against the many found by Zheng-li Shi including the 8 betacoronaviruses reported (but not published), leads me to wonder if SARS1 has been played around with a bit.
Thanks. I agree with much of this; it is a valuable analysis. However, I am less convinced by one of your opening arguments. You write “This statement clearly implied that, prior to the pandemic, WIV researchers had only ever studied a ‘short’ fragment of RaTG13”. The statement in question is: “We then found that a short region of RNA-dependent RNA polymerase (RdRp) from a bat coronavirus (BatCoV RaTG13)¬–which was previously detected in Rhinolophus affinis from Yunnan province–showed high sequence identity to 2019-nCoV. We carried out full-length sequencing on this RNA sample (GISAID accession number EPI_ISL_402131).”
To me, this statement by Shi et al could be interpreted as meaning that while only a short region of RaTG13 had high sequence identity to 2019-nCoV, they had characterised much or all of the genome.
Very interesting thank you. I’d speculate that if your theory is correct, one possibility for the soft pedaling going on is that the GOF bread crumb trail leading from WIV to Fauci and other US actors is too strong for comfort. If the US could lay this cleanly off on China, they would, but facts seem to run counter. This story is interesting and has a few good links: https://thenationalpulse.com/exclusive/white-house-visitor-logs-fauci-klain-gain-of-function/
Thanks for explaining all of this in a way that even a non-specialist can understand!
The Covid fable of 2020 was and is a nasty lie- all of it from top to bottom. All of the exaggerated and repeated fear-based messages from the media and government agencies this past year were just part of the advertising campaign for the pharmaceutical industry’s newest lucrative product- mRNA “vaccines” which they plan to roll out as new medical “miracle cures” for a variety of “ailments”, e.g. new line of “cancer vaccines.”
Every single one of the media bullhorns that were blasting the Covid fiction for the past year have direct financial ties to Pharma Inc.
It’s not exactly accurate to say Pharma uses its power to influence the news- more to the point it is Pharma that writes the script. Pharma and it’s investors own “the news” and you will speak the Pharma gospel if you wish to maintain your position of privilege- it goes without saying.
And when we speak about Pharma this cannot be separated out from the investor class who rakes in oceans of taxpayer wealth through numerous Pandemic Ponzi Schemes with Pharma as it’s “offering” and its Trojan Horse.
Covid-19 is a business plan not a medical situation.
Now more than ever it’s important to understand the economics of vaccines. Traditional systems of vaccine procurement and distribution are rapidly giving way to vast public–private supply chains. Despite annual revenues approaching US$1 trillion, the industry has been unable to reverse a declining rate of profit and finds itself in a perpetual state of crisis.
In Fall/Winter of 2019 all stocks of Big Pharma companies were in steep decline. This changed dramatically in Spring 2020 as we know.
The experimental gene therapy mRNA drugs are set to become the most lucrative drugs in medical history. Pharma depends on its “blockbuster drugs” to remain profitable and appealing to investors. It is not an exaggeration to say that without these “blockbuster drugs” the Pharma industry implodes.
What happened in 2020 was simply that the flu was re-branded and repurposed- the term Covid-19 was invented to turn the seasonal virus into the equivalent of the mythical “Al-Qaeda” meaning to terrorize the population with the false construct of a boogeyman.
This was done as the Western economies were in total free fall in late 2019 and the plan to digitize the economies of The West had to be rushed through lest the collapse be seen for what it was- the by-product of decades of Ponzi schemes by the Wall St. parasites and their allies.
The Virus™ is superfluous, just like the Swine Flu scamdemic of 2009. All they needed to do was create the perception of a pandemic/mass panic with staged Hollywood productions, doomsday models, and the fraudulent PCR test to manufacture the cases/falsely attributed as “Covid deaths.”
Covid-19 deaths follow the same age/risk stratification and seasonal curve of flu/pneumonia at the same time that flu inexplicably vanished from the face of the earth while all pneumonia deaths were suddenly called “Covid” using mass death certification fraud.
Without the fear-mongering propaganda campaign no one would’ve noticed anything unusual about the last two flu seasons because there was nothing unusual, except for the criminal, murderous, and utterly unscientific lockdown policies in response to the non- existent threat.
The only “bio-weapons” to be worried about are the poisonous injections that have already been plunged into the bloodstream of millions of people, maiming and murdering countless thousands, including perfectly healthy people who face no risk from The Virus™ and now kids have been placed in the cross hairs of Operation Covidius.
Below is a comment from Sanjoy Mahajan which was prompted by a comment from Off Guardian.
I won’t link to the OG twitter comment as the site seems to hold up comments with two links. Mahajan’s comment is cited.
OG Comment from their twitter account:
“Worth emphasizing this. The covid narrative is legitimizing flagrant fascism worldwide.
Any alt media NOT calling this out is gatekeeping – either intentionally or through pure stupidity or cowardice.”
Sanjoy Mahajan responds:
“The two enclosed tweets are true and important. Another disinformation method is at work too, seen in the omnipresent coverage of the Wuhan bioweapons lab, gain-of-function research, Fauci’s funding of it, etc. Even intelligent alt media is falling for this piece of the psyop.
All that coverage (1) strengthens the belief that viruses are contagious and deadly and can be made more so, preparing us for worse pandemics and lockdowns, and (2) distracts with talk of alleged bioweapons “over there” from the real bioweapons going into arms here.
A further but related result is to distract from the fraudulence of the whole “pandemic” conjured up with a research tool, PCR, that has been repurposed into an impossible-to-calibrate “test” made without any isolated virus and run at a Ct so high as to detect RNA fragments.
Yet I see far more coverage on, say, Children’s Health Defense about Wuhan etc. than about the PCR fraud. On Mercola’s site, it is not quite as bad but similar.
And Tucker Carlson, the only person on mainstream news supporting medical freedom (making him, in current parlance, an anti-vaxxer), has joined the Wuhan choir (along with Naomi Wolf).”
I agree with you Allen. And, after reading an article title about a rare bird flu transmission to a human in China, it becomes even more clear that there are a lot of red herrings out there. People need to put aside any thought of the virus, and look at the political climate in the world. The vaccine push is very concerning. Never before have we urged people who already had an illness to receive a vaccine.
As I understand it…
1. A virus very closely related to SARS-Cov2 WAS actually PHYSICALLY discovered in 2013 in fecal samples from bats in Yunnan Province in southwest China by Shi Zhengli from the horseshoe bat Rhinolophus affinis (RA). It was a SARS-like coronavirus (SL-CoV).
2. This virus discovered in RA in 2013, was sent to the Invitrogen lab in San Diego where a section of its genome, the RdRp section, was sequenced initially. (The purpose of this initial RdRp sequencing, was to compare the new virus with the database of known viruses.)
3. The samples sequenced in San Diego had multiple viruses in them. 2 (two) samples were BETA-coronaviruses (called HiBtCoV/3740-2 and RaBtCoV/4991) and 150 were ALPHA-coronaviruses.
4. Based on the RdRp sequencing, these viruses were sent to a centralized database called GenBank and entered there. Accession numbers (identifying numbers) were given to each sample at GenBank. (I gather that this data repository GenBank is a way that the global virology community keeps track of its many viruses.)
5. These 2 Beta-coronaviruses were unusual in 2 ways: 1) They show fairly high homology with SL-CoV, but, 2) they are NOT very similar to SARS-CoV-Classic.
Only point 1 is fully correct. No sequences were sent to San Diego, to my knowledge.
Hannah Arendt stated: “Totalitarian propaganda establishes a world fit to compete with the real one, whose main handicap is that it is not logical, consistent, and organized.”
That’s precisely what the Covid Cult is: it competes with the real world.
It’s clear what happened in 2020 if you consider the chronology.
The key week came in late January when the Chinese went public about the ´Wuhan Virus’ & CEPI (meeting in Davos at the WEF) called for international funding for vaccines even though only 20 dead at that time.
From then on we were on the road towards lockdowns, “social distancing” and other restrictions. Vaccines became the Holy Grail, other treatments were ignored. And to ensure high take up and roll out of the WEF’s Great Reset the restrictions had to continue throughout the year, even in the summer.
Also the figures had to be inflated by reclassification of deaths and ramped up use of PCR tests which gave out high number of false positives. Masks were also mandated in the summer to keep the levels of fear up.
We could and should have been back to normal many moons ago and indeed never needed lockdowns in the first place, but ending restrictions would not have helped with vaccine take-up or the implementation of the “Great Reset” so the lockdowns and restrictions (based on false data) continue.
12/30/19- Ophthalmologist Li Wenliang informed professional colleagues by WhatsApp that thereare 7 cases in his hospital that are confirmed positive for SARS.
1/1/20- Christian Drosten from the Charité heard about it and immediately started thedevelopment of SARS viruses before it was even clear or could be clear whether the report from Chinaabout SARS was true and proven before the Chinese virologists published their results. Hetestified that as of January 1, 2020, he had developed a genetic detection method to reliably prove the presence of the new corona virus in humans.
1/21/20- 3 days before the first publication of the Chinese Center for Disease Control and Prevention the WHO recommended all nations to use the “safe” test procedure developed by Drosten.
Fact: Drosten used scientifically NOT verified data for his rapidly globalised PRC test of the 2019-nCOV, which was renamed SARS-CoV-2 on February 7, 2020 with the participation of Drosten. The claim that he had a reliable test procedure is completely impossible.
The Drosten PCR test is the foundational protocol for approximately 75% of PCR tests currently being used. In the original Drosten protocol 45 cycles were recommended.
1/23/20- Publication of the development of the test method of Drosten. On page 3 of this article, left column, 8 lines from below, he describes the first and decisive step of his approach:
“Prior to the announcement of public virus sequences from 2019-nCoV cases, we relied on reports in the social media announcing the detection of a SARS-like virus. Therefore, we assumed that a SARS-relatedCoV was involved in the outbreak.”
1/24/20 and 3/220- The authoritative virologists of the Chinese disease authority published their results on 1/24/20 and 2/3/20. They reported the isolation of “many” short gene sequences, which, when strung together, could represent a genetic strand of a new type of virus.
The authors explicitly pointed out (as well as many other virologists involved to date) that the absolutely necessary experiments have not yet been carried out which would make it possible to claim that this is indeed a genetic strand of a pathogenic virus.
Keep in mind that the PCR protocol developed by Drosten is the head of the snake. Once the PCR tests are invalidated the entire swindle dissipates and withers away. Until this happens the “casedemic” can remain in perpetuity.
Keep in mind that Drosten was directly involved in virtually the same manner in the swine flu hoax of 2009- he is the German analog to the UK’s modelling madman Neil Ferguson who, like Drosten, is wrong on all well-funded (Cui Bono?) predictions which have caused incalculable damage to the general public.
The governments of The West know full well that “Covid” is being used as cover for crashing the economies in the Western world. The collapse started in 2008 and attempts to salvage this Leviathan have been failures only delaying the inevitable. Fast forward to 2019 and the crisis began to unravel again.
There is not now and never has been a “pandemic”- that is all Kabuki theater to disguise the reality of rapid economic decline brought on by the Ponzi Schemes of financial institutions over the past few decades.
The seasonal coronavirus was rebranded as Covid 19 on the back of a redefinition of what a ‘pandemic’ was by the WHO at the bequest of its financiers. This unlocked access to financial incentive programs that were large enough to bail out every failing economy. These bailouts do not come without costs.
The IMF and World Bank give you whatever money you needed to ‘support’ your country through the crisis on condition of- You do what we say, you test, track and chip everyone, you tell our story via your media and most importantly you pay us back at some time in the future and that payback will likely be a currency reset and global governance.
Covid 19 is the final heist to strip all value out of the world in an effort by the creditors to consolidate what they believe they’re owed.
We are all collateral damage.
Thank you for responding Jonathan.
I am sure you must know that Shi and Baric from The University of North Carolina at Chapel Hill were working together during that period and published their GOF virus results in Nature Med in 2015.
There are more than one examples pointing to the fact that the US and possibly other labs around the world were working on the same GOF of the same or similar strains. Until this is addressed it just looks like a concerted effort to blame China China CHina.
Dr George Gao as head of Chinese CDC and the 2012 Miners Phd Supervisor seems a key and central player in covering up both of what tests were done on the 2012 miners and what data/samples is/are available.
Dr Gao also covered up in
real time the covid data in Dec 2019 and early 2020
It is clear that Dr Gao knew something very serious was going on as by the 27th Dec 2019 the health authorities had receieved a near-complete sequence of the virus, showing a SARS coronavirus
By the 31st of December 2019 Dr Gao told at least one important US researcher he [Dr Gao] had had confirmation there was a new coronavirus in Wuhan
and by the 3rd Jan Dr Gao new this was a fully sequenced and lab confirmed as new SARS
Its UNREAL but if you watch this PBS documentary linked below from 20mins and 05 seconds you will see
Peter Daszak interviewed saying he was in contact with George Gao on the 31st Dec 2019 about the Wuhan Virus outbreak offering help with the outbreak and
next an interview with US Professor Ian Lipkin who says George Gao contacted him on the 31st Dec 2019 with Lipkin saying he was told by Dr Gao of the lab confirmation of a new coronavirus in Wuhan
From around 11 mins 50 seconds you will see the that on the 27th Dec a Chinese lab had already confirmed a SARS like coronavirus and notified the health authorities
It is interesting that the Chinese have never denied these events happened as set out or that the media does not ask Dr Gao or the Chinese about this
We know Dr George Gao was the supervisor of the Phd on the 2012 miners, head of the Chinese CDC and a top Covid advisor to President Xi
So before the 1st Jan 2020 Dr Gao was at the center of the Chinese response and controling the information and public statements about Covid
It is therefore all but certain he was also centrally involved in the later responses to the 2012 miners data questions
In any event it is not reasonable to say that China did not later absolutely control what data was released on the 2012 Miners or that it does not continue to fail to provide samples and full background on what tests were done or not done or calrification about the full results speaks for itself
It seems very clear from the limited data that is available that a coronavirus was detected in 2012 miners samples, and that China continues to be economical with the truth.
It is clearly very possible that the 2012 miners coronavirus was a SARS type virus
That the Chinese have not resolved this by releasing samples and full data on the tests, during the repeated and very belated and unclear or misleading updates on the 2012 events, means they are covering up something.
Dr Gao withheld information in 2019 and early 2020 from the WHO and the world on Covid as by 3rd of Jan 2020 he had had full lab confirmation there was a new SARS coronavirus Wuhan.
President Xi is also another central player in all this as he publically stated in a Feb. 3 speech to China’s most powerful leaders that he [Presid Xi] had “continuously given verbal and written instructions” since Jan. 7 2019
George Gao Covid Timeline in public domain
Dec. 27: A Chinese lab assembles a near-complete sequence of the virus, showing a SARS coronavirus like that of the 2002-03 SARS outbreak. The lab alerts health authorities, but the information is kept under wraps.
— Dec. 30: Doctors begin warning about the SARS outbreak independently on social media — share a lab report indicating the pathogen is a SARS virus and warn to wear protective clothing against human to human transmission
31 Dec 2019 George Gao head of Chinese CDC discusses Wuhan Virus with US Professor Lipkin and confirms it is a coronavirus plus has limited contact with Peter Daszak on the Wuhan virus event
Jan. 3, 2020: The Chinese Center for Disease Control and Prevention obtains a full sequence of the virus but doesn’t release it.
China reports a virus outbreak to the WHO but not that its confirmed as a SARS coronavirus
3rd of Jan 2020 the National CCP and Chinese CDC ordered destruction of Covid-19 patient samples the same day as Dr Gao warned the US CDC by phone that there was a major problem in Wuhan but gave no data
3 January Robert Redfield, director of the US Centers for Disease Control and Prevention (CDC), spoke with George Gao, and was alerted to the newly emerging disease
Either the destroyed virus samples were very dangerous or China was hiding the SARS outbreak – either way it was a cover up by the Beijing CCP Government.
Human to human transmission was also confirmed by social media as medical staff were getting infected from patients
Liu Dengfeng, a supervisor at the science and education division of China’s National Health Commission, confirmed it was a Beijing Chinese Central CCP government that issued orders on January 3 to labs and medical institutes to destroy Covid-19 patient samples
7th Jan President Xi in full control and issuing instructions on Covid-19 he must therefore have been briefed for some time by George Gao on Wuhan SARS
Jan. 11: WHO says Chinese investigators have conducted gene sequencing of the virus
Jan. 13: The first case outside China is identified in Thailand.
Also see data in pdf below referred to in another post on this site. It seems well done but I have not idea if the claims fully or partly stack up.
Do you have any evidence to support your points 2-5?
On 16 February 2020 Nextstrain.org tweeted a phylogenetic tree that had an antecedent branch to that of the Wuhan strains, leading off from around early December. An example of this strain was detected in the US on or around January 29. I always wondered what that represented.
Adam, you comment seems to indicate that Covid-19 was or is actually a big problem. There is not mathematical evidence to back that up. In 2020, at least 50 million people died worldwide and roughly 2 million of those deaths were blamed on Covid-19. That right there should tell everyone that something else is going on. I had the exact symptoms of Covid-19, right down to the loss of taste and smell, 3.5 years ago. When I mention that, nobody has any sort of an explanation. My husband recently met a nurse who believes this virus has been around a long time. She quit her job after they had a stage 4 lung cancer patient die and the hospital coded it as a Covid-19 death. My cousin works weekends in an ER and they were told to code every death as Covid-19, except decapitation. The PCR test was never meant do be used as a diagnostic tool. There is definitely something going on, but it does not appear to be a deadly virus.
Didn’t proofread carefully. That should read “your comment”. And “no mathematical evidence”. Geeze, and I’ve already had coffee today! Kind of hard to make a valid point when I can’t type my ideas correctly!
We already knew this from a year now. That was all multimillion dollar politics involving international organizations, governments and media such press and social media worldwide. They all conspired to keep this shut. The link to scientific American cites Daszak many times. Independent science news deserves a Nobel Prize.